Hotspot

rps16

LSC · 5271-6368 · 1098 bp

Back to Canna

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1098
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TGAATTACACTGCACGACCC TACTGCCCGTTGATCCAATG 50.0 50.0 58.2 58.0 1945 bp 1.0 78.1
rps16_p2 ACTGCACGACCCCATTAAAA TACTGCCCGTTGATCCAATG 45.0 50.0 57.7 58.0 1937 bp 1.0 77.5
rps16_p3 TGAATTACACTGCACGACCC TCGTCCCGAACAATCTGTTG 50.0 50.0 58.2 58.2 2125 bp 1.0 76.8
rps16_p4 ACTGCACGACCCCATTAAAA TCGTCCCGAACAATCTGTTG 45.0 50.0 57.7 58.2 2117 bp 1.0 76.1
rps16_p5 TGAATTACACTGCACGACCC TGATCGTCCCGAACAATCTG 50.0 50.0 58.2 57.7 2128 bp 1.0 76.1