Hotspot

psbA

LSC · 307-1368 · 1062 bp

Back to Canna

Pi 0
MarkerSeek score 40.00
Primer available yes
Label psbA

Feature Metadata

label_name
psbA
feature_type
gene
region
LSC
length_bp
1062
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA_p1 ACAAGATAGGGGGTATTGCT AATACGTGTGCTTGGGAGTC 45.0 50.0 55.6 57.9 1185 bp 1.0 59.8
psbA_p2 AACAAGATAGGGGGTATTGCT AATACGTGTGCTTGGGAGTC 42.9 50.0 56.4 57.9 1186 bp 1.0 58.3
psbA_p3 ACAAGATAGGGGGTATTGCT GTGTGCTTGGGAGTCCTTAA 45.0 50.0 55.6 57.4 1180 bp 1.0 56.0
psbA_p4 ACAAGATAGGGGGTATTGCTAC AATACGTGTGCTTGGGAGTC 45.5 50.0 57.0 57.9 1185 bp 1.0 55.4
psbA_p5 AACAAGATAGGGGGTATTGCT GTGTGCTTGGGAGTCCTTAA 42.9 50.0 56.4 57.4 1181 bp 1.0 54.5