Hotspot

atpI

LSC · 17239-17982 · 744 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 AACCACGCATGAATTGGGAT GAGTCGGTGAGTTGAGAAAGA 45.0 47.6 58.1 57.4 900 bp 1.0 65.7
atpI_p2 AACCACGCATGAATTGGGAT GTCGGTGAGTTGAGAAAGAGA 45.0 47.6 58.1 57.4 898 bp 1.0 65.7
atpI_p3 AACCACGCATGAATTGGGAT AGAGTCGGTGAGTTGAGAAAG 45.0 47.6 58.1 57.3 901 bp 1.0 65.6
atpI_p4 AACCACGCATGAATTGGGAT AGTCGGTGAGTTGAGAAAGAG 45.0 47.6 58.1 57.3 899 bp 1.0 65.6
atpI_p5 AACCACGCATGAATTGGGAT AGTCGGTGAGTTGAGAAAGA 45.0 45.0 58.1 56.1 899 bp 1.0 63.9