Hotspot

atpA

LSC · 11983-13506 · 1524 bp

Back to Canna

Pi 0
MarkerSeek score 40.00
Primer available yes
Label atpA

Feature Metadata

label_name
atpA
feature_type
gene
region
LSC
length_bp
1524
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpA_p1 CGAATCAGAACTATTTCTCAACGA CTCGGGACCATGCAAGAAAT 37.5 50.0 57.1 58.2 1688 bp 1.0 40.0
atpA_p2 TCGAATCAGAACTATTTCTCAACG CTCGGGACCATGCAAGAAAT 37.5 50.0 57.1 58.2 1689 bp 1.0 40.0
atpA_p3 CGAATCAGAACTATTTCTCAACGA ATATTGGCATTCTCGGGACC 37.5 50.0 57.1 57.4 1699 bp 1.0 40.0
atpA_p4 TCGAATCAGAACTATTTCTCAACG ATATTGGCATTCTCGGGACC 37.5 50.0 57.1 57.4 1700 bp 1.0 40.0
atpA_p5 CGAATCAGAACTATTTCTCAACG CTCGGGACCATGCAAGAAAT 39.1 50.0 55.8 58.2 1688 bp 1.0 40.0