Hotspot

trnS-GCU-trnS-CGA

LSC · 9297-10886 · 1590 bp

Back to Canna

Pi 0.0113
MarkerSeek score 65.30
Primer available yes
Label trnS-GCU-trnS-CGA

Feature Metadata

label_name
trnS-GCU-trnS-CGA
feature_type
igs
region
LSC
length_bp
1590
variable_sites
18
pi
0.0113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnS-CGA_p1 TCAAGTAATCAGAGCGAGCG AACTGAGAGACCCCTTAACT 50.0 45.0 57.8 55.1 1764-1771 bp 1.0 56.0
trnS-GCU-trnS-CGA_p2 CGGAGAGAGAGGGATTCGAA AACTGAGAGACCCCTTAACT 55.0 45.0 58.3 55.1 1746-1753 bp 1.0 55.0
trnS-GCU-trnS-CGA_p3 AGCAATCCGACGCTTTAGTC AACTGAGAGACCCCTTAACT 50.0 45.0 58.4 55.1 1695-1702 bp 1.0 54.7
trnS-GCU-trnS-CGA_p4 TCAAGTAATCAGAGCGAGCG AAACTGAGAGACCCCTTAACT 50.0 42.9 57.8 55.9 1765-1772 bp 1.0 54.6
trnS-GCU-trnS-CGA_p5 CTTTAGTCCGCTCAGCCATC AACTGAGAGACCCCTTAACT 55.0 45.0 58.4 55.1 1683-1690 bp 1.0 54.2