Hotspot

trnK-UUU-rps16

LSC · 4470-5270 · 801 bp

Back to Canna

Pi 0
MarkerSeek score 40.00
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
801
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TGGACGAATTTGGTGCTTCA TCAACCAACAGGAACCGTTT 45.0 45.0 58.0 57.9 958 bp 1.0 78.7
trnK-UUU-rps16_p2 TGGACGAATTTGGTGCTTCA CTCAACCAACAGGAACCGTT 45.0 50.0 58.0 58.3 959 bp 1.0 77.2
trnK-UUU-rps16_p3 TCTGGACGAATTTGGTGCTT TCAACCAACAGGAACCGTTT 45.0 45.0 57.7 57.9 960 bp 1.0 76.8
trnK-UUU-rps16_p4 TCTGGACGAATTTGGTGCTT CTCAACCAACAGGAACCGTT 45.0 50.0 57.7 58.3 961 bp 1.0 75.2
trnK-UUU-rps16_p5 GTCTGGACGAATTTGGTGCT TCAACCAACAGGAACCGTTT 50.0 45.0 58.5 57.9 961 bp 1.0 75.1