Hotspot

matK-trnK-UUU

LSC · 3503-4431 · 929 bp

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Pi 0
MarkerSeek score 40.00
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
929
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TGTTGTTGCCGAGATCCATT GGGTCGTGCAGTGTAATTCA 45.0 50.0 57.8 58.2 1093 bp 1.0 76.8
matK-trnK-UUU_p2 AGAAAGTGTTGTTGCCGAGA GGGTCGTGCAGTGTAATTCA 45.0 50.0 57.7 58.2 1099 bp 1.0 75.8
matK-trnK-UUU_p3 AAGTGTTGTTGCCGAGATCC GGGTCGTGCAGTGTAATTCA 50.0 50.0 58.5 58.2 1096 bp 1.0 74.6
matK-trnK-UUU_p4 GTGTTGTTGCCGAGATCCAT GGGTCGTGCAGTGTAATTCA 50.0 50.0 58.5 58.2 1094 bp 1.0 74.1
matK-trnK-UUU_p5 TGTTGTTGCCGAGATCCATT TAATGGGGTCGTGCAGTGTA 45.0 50.0 57.8 58.7 1098 bp 1.0 72.6