primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	ACAAGATAGGGGGTATTGCT	AATACGTGTGCTTGGGAGTC	20	20	45.0	50.0	55.6	57.9	0.0	0.0	2.530992	307	1368	1185-1185	1.000000	59.8
psbA_p2	psbA	2	AACAAGATAGGGGGTATTGCT	AATACGTGTGCTTGGGAGTC	21	20	42.9	50.0	56.4	57.9	0.0	0.0	2.715682	307	1368	1186-1186	1.000000	58.3
psbA_p3	psbA	3	ACAAGATAGGGGGTATTGCT	GTGTGCTTGGGAGTCCTTAA	20	20	45.0	50.0	55.6	57.4	0.0	9.6	2.997708	307	1368	1180-1180	1.000000	56.0
psbA_p4	psbA	4	ACAAGATAGGGGGTATTGCTAC	AATACGTGTGCTTGGGAGTC	22	20	45.5	50.0	57.0	57.9	0.0	0.0	3.071303	307	1368	1185-1185	1.000000	55.4
psbA_p5	psbA	5	AACAAGATAGGGGGTATTGCT	GTGTGCTTGGGAGTCCTTAA	21	20	42.9	50.0	56.4	57.4	0.0	9.6	3.182398	307	1368	1181-1181	1.000000	54.5
matK-trnK-UUU_p1	matK-trnK-UUU	1	TGTTGTTGCCGAGATCCATT	GGGTCGTGCAGTGTAATTCA	20	20	45.0	50.0	57.8	58.2	0.0	0.0	0.395493	3503	4431	1093-1093	1.000000	76.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	AGAAAGTGTTGTTGCCGAGA	GGGTCGTGCAGTGTAATTCA	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.522249	3503	4431	1099-1099	1.000000	75.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	AAGTGTTGTTGCCGAGATCC	GGGTCGTGCAGTGTAATTCA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.672908	3503	4431	1096-1096	1.000000	74.6
matK-trnK-UUU_p4	matK-trnK-UUU	4	GTGTTGTTGCCGAGATCCAT	GGGTCGTGCAGTGTAATTCA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.743511	3503	4431	1094-1094	1.000000	74.1
matK-trnK-UUU_p5	matK-trnK-UUU	5	TGTTGTTGCCGAGATCCATT	TAATGGGGTCGTGCAGTGTA	20	20	45.0	50.0	57.8	58.7	0.0	0.0	0.930761	3503	4431	1098-1098	1.000000	72.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	TGGACGAATTTGGTGCTTCA	TCAACCAACAGGAACCGTTT	20	20	45.0	45.0	58.0	57.9	4.8	0.0	0.157511	4470	5270	958-958	1.000000	78.7
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TGGACGAATTTGGTGCTTCA	CTCAACCAACAGGAACCGTT	20	20	45.0	50.0	58.0	58.3	4.8	0.0	0.350183	4470	5270	959-959	1.000000	77.2
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TCTGGACGAATTTGGTGCTT	TCAACCAACAGGAACCGTTT	20	20	45.0	45.0	57.7	57.9	4.8	0.0	0.402128	4470	5270	960-960	1.000000	76.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	TCTGGACGAATTTGGTGCTT	CTCAACCAACAGGAACCGTT	20	20	45.0	50.0	57.7	58.3	4.8	0.0	0.594800	4470	5270	961-961	1.000000	75.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	GTCTGGACGAATTTGGTGCT	TCAACCAACAGGAACCGTTT	20	20	50.0	45.0	58.5	57.9	4.8	0.0	0.608018	4470	5270	961-961	1.000000	75.1
rps16_p1	rps16	1	TGAATTACACTGCACGACCC	TACTGCCCGTTGATCCAATG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.233623	5271	6368	1945-1945	1.000000	78.1
rps16_p2	rps16	2	ACTGCACGACCCCATTAAAA	TACTGCCCGTTGATCCAATG	20	20	45.0	50.0	57.7	58.0	0.0	0.0	0.316892	5271	6368	1937-1937	1.000000	77.5
rps16_p3	rps16	3	TGAATTACACTGCACGACCC	TCGTCCCGAACAATCTGTTG	20	20	50.0	50.0	58.2	58.2	0.0	0.0	0.405911	5271	6368	2125-2125	1.000000	76.8
rps16_p4	rps16	4	ACTGCACGACCCCATTAAAA	TCGTCCCGAACAATCTGTTG	20	20	45.0	50.0	57.7	58.2	0.0	0.0	0.489179	5271	6368	2117-2117	1.000000	76.1
rps16_p5	rps16	5	TGAATTACACTGCACGACCC	TGATCGTCCCGAACAATCTG	20	20	50.0	50.0	58.2	57.7	0.0	0.0	0.490343	5271	6368	2128-2128	1.000000	76.1
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAACAGAAAAATGGGGCGTG	20	20	45.0	50.0	58.1	57.9	8.1	0.0	0.203897	6369	7865	1629-1629	1.000000	78.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	AAGTCGCACGTTGCTTTCTA	GTGCCGAACAGAAAAATGGG	20	20	45.0	50.0	58.1	57.9	8.1	0.0	0.203897	6369	7865	1634-1634	1.000000	78.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6369	7865	1570-1570	1.000000	78.3
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TCATGTCCTTCAAGTCGCAC	GAACAGAAAAATGGGGCGTG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.268806	6369	7865	1640-1640	1.000000	77.8
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TCATGTCCTTCAAGTCGCAC	GTGCCGAACAGAAAAATGGG	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.268806	6369	7865	1645-1645	1.000000	77.8
trnS-GCU-trnS-CGA_p1	trnS-GCU-trnS-CGA	1	TCAAGTAATCAGAGCGAGCG	AACTGAGAGACCCCTTAACT	20	20	50.0	45.0	57.8	55.1	0.0	0.0	3.130370	9297	10886	1764-1771	1.000000	56.0
trnS-GCU-trnS-CGA_p2	trnS-GCU-trnS-CGA	2	CGGAGAGAGAGGGATTCGAA	AACTGAGAGACCCCTTAACT	20	20	55.0	45.0	58.3	55.1	0.0	0.0	3.254032	9297	10886	1746-1753	1.000000	55.0
trnS-GCU-trnS-CGA_p3	trnS-GCU-trnS-CGA	3	AGCAATCCGACGCTTTAGTC	AACTGAGAGACCCCTTAACT	20	20	50.0	45.0	58.4	55.1	0.0	0.0	3.293993	9297	10886	1695-1702	1.000000	54.7
trnS-GCU-trnS-CGA_p4	trnS-GCU-trnS-CGA	4	TCAAGTAATCAGAGCGAGCG	AAACTGAGAGACCCCTTAACT	20	21	50.0	42.9	57.8	55.9	0.0	0.0	3.294177	9297	10886	1765-1772	1.000000	54.6
trnS-GCU-trnS-CGA_p5	trnS-GCU-trnS-CGA	5	CTTTAGTCCGCTCAGCCATC	AACTGAGAGACCCCTTAACT	20	20	55.0	45.0	58.4	55.1	0.0	0.0	3.359214	9297	10886	1683-1690	1.000000	54.2
trnS-CGA_p1	trnS-CGA	1	TCGCTCCTTTGTTCGACAAA	TGCATATCTAAATTGATGGTGCA	20	23	45.0	34.8	58.1	56.6	11.1	12.5	4.423978	10887	11664	879-879	1.000000	44.6
trnS-CGA_p2	trnS-CGA	2	TTCGCTCCTTTGTTCGACAA	TGCATATCTAAATTGATGGTGCA	20	23	45.0	34.8	58.1	56.6	0.0	12.5	4.423978	10887	11664	880-880	1.000000	44.6
trnS-CGA_p3	trnS-CGA	3	TTTCGCTCCTTTGTTCGACA	TGCATATCTAAATTGATGGTGCA	20	23	45.0	34.8	58.1	56.6	0.0	12.5	4.423978	10887	11664	881-881	1.000000	44.6
trnS-CGA_p4	trnS-CGA	4	TGATTGCCTTTCGCTCCTTT	TGCATATCTAAATTGATGGTGCA	20	23	45.0	34.8	58.1	56.6	0.0	12.5	4.456955	10887	11664	889-889	1.000000	44.3
trnS-CGA_p5	trnS-CGA	5	TTGATTGCCTTTCGCTCCTT	TGCATATCTAAATTGATGGTGCA	20	23	45.0	34.8	58.1	56.6	0.0	12.5	4.456955	10887	11664	890-890	1.000000	44.3
atpA_p1	atpA	1	CGAATCAGAACTATTTCTCAACGA	CTCGGGACCATGCAAGAAAT	24	20	37.5	50.0	57.1	58.2	0.0	0.0	5.115865	11983	13506	1688-1688	1.000000	40.0
atpA_p2	atpA	2	TCGAATCAGAACTATTTCTCAACG	CTCGGGACCATGCAAGAAAT	24	20	37.5	50.0	57.1	58.2	0.0	0.0	5.115865	11983	13506	1689-1689	1.000000	40.0
atpA_p3	atpA	3	CGAATCAGAACTATTTCTCAACGA	ATATTGGCATTCTCGGGACC	24	20	37.5	50.0	57.1	57.4	0.0	0.0	5.439667	11983	13506	1699-1699	1.000000	40.0
atpA_p4	atpA	4	TCGAATCAGAACTATTTCTCAACG	ATATTGGCATTCTCGGGACC	24	20	37.5	50.0	57.1	57.4	0.0	0.0	5.439667	11983	13506	1700-1700	1.000000	40.0
atpA_p5	atpA	5	CGAATCAGAACTATTTCTCAACG	CTCGGGACCATGCAAGAAAT	23	20	39.1	50.0	55.8	58.2	0.0	0.0	5.442038	11983	13506	1688-1688	1.000000	40.0
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	AAAAAGGGTGAGCGAAGTGA	20	20	45.0	45.0	57.4	57.7	9.6	0.0	0.983985	13580	14921	1517-1517	1.000000	72.1
atpF_p2	atpF	2	ACTAATTTCGTCGGCTCGAA	GGGTGAGCGAAGTGATACAA	20	20	45.0	50.0	57.4	57.6	9.6	0.0	1.022222	13580	14921	1512-1512	1.000000	71.8
atpF_p3	atpF	3	ACTAATTTCGTCGGCTCGAA	AGGGTGAGCGAAGTGATACA	20	20	45.0	50.0	57.4	58.4	9.6	0.0	1.085040	13580	14921	1513-1513	1.000000	71.3
atpF_p4	atpF	4	ACTAATTTCGTCGGCTCGAA	AAAAGGGTGAGCGAAGTGAT	20	20	45.0	45.0	57.4	57.4	9.6	0.0	1.210761	13580	14921	1516-1516	1.000000	70.3
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	AAGGGTGAGCGAAGTGATAC	20	20	45.0	50.0	57.4	57.3	9.6	0.0	1.316934	13580	14921	1514-1514	1.000000	69.5
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACGTTAGCCGC	20	20	45.0	45.0	58.0	58.6	1.4	16.5	0.624009	15734	17238	1594-1594	1.000000	75.1
atpH-atpI_p2	atpH-atpI	2	ATAACGGAAGCGGCAGAAAT	TAGGTGAATCCATGGAGGGT	20	20	45.0	50.0	58.0	57.4	1.4	21.3	0.625447	15734	17238	1566-1566	1.000000	75.0
atpH-atpI_p3	atpH-atpI	3	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	58.0	58.6	3.3	16.5	0.633001	15734	17238	1677-1677	1.000000	75.0
atpH-atpI_p4	atpH-atpI	4	TACCTTCTACAGCTTGGCCT	TAGGTGAATCCATGGAGGGT	20	20	50.0	50.0	58.0	57.4	3.3	21.3	0.634438	15734	17238	1649-1649	1.000000	75.0
atpH-atpI_p5	atpH-atpI	5	TACCTTGACCAACTCCAGGT	ATTTTTGCAACGTTAGCCGC	20	20	50.0	45.0	57.9	58.6	0.0	16.5	0.697901	15734	17238	1650-1650	1.000000	74.5
atpI_p1	atpI	1	AACCACGCATGAATTGGGAT	GAGTCGGTGAGTTGAGAAAGA	20	21	45.0	47.6	58.1	57.4	0.0	0.0	1.793580	17239	17982	900-900	1.000000	65.7
atpI_p2	atpI	2	AACCACGCATGAATTGGGAT	GTCGGTGAGTTGAGAAAGAGA	20	21	45.0	47.6	58.1	57.4	0.0	0.0	1.793580	17239	17982	898-898	1.000000	65.7
atpI_p3	atpI	3	AACCACGCATGAATTGGGAT	AGAGTCGGTGAGTTGAGAAAG	20	21	45.0	47.6	58.1	57.3	0.0	0.0	1.800499	17239	17982	901-901	1.000000	65.6
atpI_p4	atpI	4	AACCACGCATGAATTGGGAT	AGTCGGTGAGTTGAGAAAGAG	20	21	45.0	47.6	58.1	57.3	0.0	0.0	1.800499	17239	17982	899-899	1.000000	65.6
atpI_p5	atpI	5	AACCACGCATGAATTGGGAT	AGTCGGTGAGTTGAGAAAGA	20	20	45.0	45.0	58.1	56.1	0.0	0.0	2.010922	17239	17982	899-899	1.000000	63.9
rpl22-rps19_p1	rpl22-rps19	1	CGCGCTTTGAAAACAGACAT	CTGACTTTTGCGAGACATGC	20	20	45.0	50.0	58.0	57.8	5.6	0.0	0.274802	90320	90944	693-695	1.000000	78.0
rpl22-rps19_p2	rpl22-rps19	2	TTCGCGCTTTGAAAACAGAC	CTGACTTTTGCGAGACATGC	20	20	45.0	50.0	57.9	57.8	5.6	0.0	0.337878	90320	90944	695-697	1.000000	77.5
rpl22-rps19_p3	rpl22-rps19	3	CGCGCTTTGAAAACAGACAT	CGAGACATGCGAGAAACGAT	20	20	45.0	50.0	58.0	58.4	5.6	0.0	0.486492	90320	90944	683-685	1.000000	76.3
rpl22-rps19_p4	rpl22-rps19	4	CGCGCTTTGAAAACAGACAT	TTTGCGAGACATGCGAGAAA	20	20	45.0	45.0	58.0	58.5	5.6	0.0	0.528995	90320	90944	687-689	1.000000	76.0
rpl22-rps19_p5	rpl22-rps19	5	CGCGCTTTGAAAACAGACAT	TTTTGCGAGACATGCGAGAA	20	20	45.0	45.0	58.0	58.5	5.6	0.0	0.528995	90320	90944	688-690	1.000000	76.0
psaC-ndhE_p1	psaC-ndhE	1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	126034	127127	1230-1288	1.000000	77.7
psaC-ndhE_p2	psaC-ndhE	2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	58.1	57.4	0.0	0.0	0.753385	126034	127127	1229-1287	1.000000	77.7
psaC-ndhE_p3	psaC-ndhE	3	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	20	20	50.0	55.0	58.1	58.8	0.0	6.0	0.891951	126034	127127	1228-1286	1.000000	76.6
psaC-ndhE_p4	psaC-ndhE	4	CAGGCTCGTACACATTGAGT	CTGAAGCAGCTATTGGGCTA	20	20	50.0	50.0	57.6	57.4	0.0	0.0	0.997796	126034	127127	1190-1248	1.000000	75.8
psaC-ndhE_p5	psaC-ndhE	5	CAGGCTCGTACACATTGAGT	TGAAGCAGCTATTGGGCTAG	20	20	50.0	50.0	57.6	57.4	0.0	0.0	0.997796	126034	127127	1189-1247	1.000000	75.8