Hotspot

ndhA

SSC · 117887-120246 · 2360 bp

Back to Canavalia

Pi 0.000424

MarkerSeek score 54.70

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2360
variable_sites: 2
pi: 0.000424
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGACCATAATTCATGAACCCAGT	GTCCACCGGGTTTGATCAAT	39.1	50.0	57.8	58.2	2586-2597 bp	1.0	53.4
ndhA_p2	TGACCATAATTCATGAACCCAGT	TCCACCGGGTTTGATCAATT	39.1	45.0	57.8	57.4	2585-2596 bp	1.0	49.9
ndhA_p3	TGACCATAATTCATGAACCCAGT	CCGGGTTTGATCAATTTGCA	39.1	45.0	57.8	57.3	2581-2592 bp	1.0	48.8
ndhA_p4	TGACCATAATTCATGAACCCAGT	ACCGGGTTTGATCAATTTGC	39.1	45.0	57.8	57.3	2582-2593 bp	1.0	48.8
ndhA_p5	TGACCATAATTCATGAACCCAG	GTCCACCGGGTTTGATCAAT	40.9	50.0	56.1	58.2	2586-2597 bp	1.0	47.9