Hotspot

petA-psbJ

LSC · 63298-63704 · 407 bp

Back to Canavalia

Pi 0.00287

MarkerSeek score 61.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 407
variable_sites: 2
pi: 0.00287
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	522-559 bp	1.0	77.8
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.3	57.4	527-564 bp	1.0	77.3
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	531-568 bp	1.0	76.9
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.8	57.1	520-557 bp	1.0	75.5
petA-psbJ_p5	CGTGTCCAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	50.0	50.0	57.8	57.1	523-560 bp	1.0	75.5