Hotspot

rpoB-trnC-GCA

LSC · 54337-55467 · 1131 bp

Back to Canavalia

Pi 0.00324

MarkerSeek score 61.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1131
variable_sites: 6
pi: 0.00324
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTTCTGTTAAGCCCCGATC	CTTGTCTATCAGGCGACACC	55.0	55.0	58.0	58.1	1314-1387 bp	1.0	79.6
rpoB-trnC-GCA_p2	CCTTCTGTTAAGCCCCGATC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.0	58.1	1295-1368 bp	1.0	79.5
rpoB-trnC-GCA_p3	AACCCCGGTAGTGTAGACAT	CTTGTCTATCAGGCGACACC	50.0	55.0	57.8	58.1	1257-1330 bp	1.0	78.0
rpoB-trnC-GCA_p4	ACCCCGGTAGTGTAGACATT	CTTGTCTATCAGGCGACACC	50.0	55.0	57.8	58.1	1256-1329 bp	1.0	78.0
rpoB-trnC-GCA_p5	ACCCCGGTAGTGTAGACATT	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1237-1310 bp	1.0	78.0