Hotspot

trnS-GCU-trnG-UCC

LSC · 35705-36053 · 349 bp

Back to Canavalia

Pi 0.00334

MarkerSeek score 56.40

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 349
variable_sites: 2
pi: 0.00334
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TAAGGGGTCCATGGAACGAA	50.0	50.0	58.0	58.3	432-433 bp	1.0	78.0
trnS-GCU-trnG-UCC_p2	CAATCCGACGCTTTAGTCCA	TTAAGGGGTCCATGGAACGA	50.0	50.0	58.0	58.3	433-434 bp	1.0	78.0
trnS-GCU-trnG-UCC_p3	AATCCGACGCTTTAGTCCAC	TAAGGGGTCCATGGAACGAA	50.0	50.0	58.0	58.3	431-432 bp	1.0	78.0
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	TTAAGGGGTCCATGGAACGA	50.0	50.0	58.0	58.3	432-433 bp	1.0	78.0
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	TAAGGGGTCCATGGAACGAA	50.0	50.0	58.2	58.3	485-486 bp	1.0	76.7