Hotspot

trnQ-UUG-psbK

LSC · 34279-34818 · 540 bp

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Pi 0.0107

MarkerSeek score 69.50

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 540
variable_sites: 9
pi: 0.0107
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	CTGGATATGTTCTGGGACGG	AGGCTTCAGGTAATTTCGCC	55.0	50.0	57.5	58.2	686-744 bp	1.0	77.9
trnQ-UUG-psbK_p2	ATATGTTCTGGGACGGGAGG	AGGCTTCAGGTAATTTCGCC	55.0	50.0	58.6	58.2	682-740 bp	1.0	77.6
trnQ-UUG-psbK_p3	GGATATGTTCTGGGACGGGA	AGGCTTCAGGTAATTTCGCC	55.0	50.0	58.6	58.2	684-742 bp	1.0	77.6
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCC	50.0	50.0	58.7	58.2	641-699 bp	1.0	77.0
trnQ-UUG-psbK_p5	GATATGTTCTGGGACGGGAG	AGGCTTCAGGTAATTTCGCC	55.0	50.0	57.2	58.2	683-741 bp	1.0	75.6