Hotspot

clpP

LSC · 73303-75498 · 2196 bp

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Pi 0.0326

MarkerSeek score 66.00

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2196
variable_sites: 96
pi: 0.0326
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TGACCCTGTTAGTCCGTTCT	GCCATTAGTACGCTTCCACA	50.0	50.0	58.0	58.0	2732-2947 bp	0.666667	73.3
clpP_p2	CCCCAAGAGCAGGAGATTTC	GCCATTAGTACGCTTCCACA	55.0	50.0	58.0	58.0	2370-2585 bp	0.666667	73.3
clpP_p3	CTGATTGGCTGTCTTGGGTT	GCCATTAGTACGCTTCCACA	50.0	50.0	58.1	58.0	2341-2556 bp	0.666667	73.1
clpP_p4	ATGAAGCTTGGTTGGGTAGC	GCCATTAGTACGCTTCCACA	50.0	50.0	58.2	58.0	2752-2967 bp	0.666667	72.1
clpP_p5	CCTGTTAGTCCGTTCTTGCA	GCCATTAGTACGCTTCCACA	50.0	50.0	57.8	58.0	2728-2943 bp	0.666667	72.1