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ndhF-rpl32

SSC · 120853-121922 · 1070 bp

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Pi 0.081

MarkerSeek score 77.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1070
variable_sites: 92
pi: 0.081
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	ACATAGGAACCGGAAGTGGA	ACGAATACGCTTCTTGGAGA	50.0	45.0	58.1	56.7	749-1179 bp	1.0	80.4
ndhF-rpl32_p2	AACCGGAAGTGGAACGAAAG	ACGAATACGCTTCTTGGAGA	50.0	45.0	58.4	56.7	742-1172 bp	1.0	77.6
ndhF-rpl32_p3	GAACCGGAAGTGGAACGAAA	ACGAATACGCTTCTTGGAGA	50.0	45.0	58.4	56.7	743-1173 bp	1.0	77.6
ndhF-rpl32_p4	CGGAAGTGGAACGAAAGGTA	ACGAATACGCTTCTTGGAGA	50.0	45.0	57.6	56.7	739-1169 bp	1.0	77.4
ndhF-rpl32_p5	ACATAGGAACCGGAAGTGGA	ACGAATACGCTTCTTGGAGAT	50.0	42.9	58.1	57.2	749-1179 bp	1.0	76.5