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rps12-clpP

LSC · 73043-73302 · 260 bp

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Pi 0.0576

MarkerSeek score 63.40

Primer available yes

Label rps12-clpP

Feature Metadata

label_name: rps12-clpP
feature_type: igs
region: LSC
length_bp: 260
variable_sites: 13
pi: 0.0576
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP_p1	CCCCAAGAGCAGGAGATTTC	TATAAAGCAACGGGGCCATC	55.0	50.0	58.0	58.0	752-826 bp	1.0	87.5
rps12-clpP_p2	TGACCCTGTTAGTCCGTTCT	GAATTCCATTGGAGAGCCGT	50.0	50.0	58.0	58.0	973-1047 bp	1.0	86.7
rps12-clpP_p3	TGACCCTGTTAGTCCGTTCT	TATAAAGCAACGGGGCCATC	50.0	50.0	58.0	58.0	1114-1188 bp	1.0	86.6
rps12-clpP_p4	TGACCCTGTTAGTCCGTTCT	ACGAACAGACAAGCCCTTTT	50.0	45.0	58.0	57.9	810 bp	0.333333	52.9
rps12-clpP_p5	TGACCCTGTTAGTCCGTTCT	ACTACTGAAACTGCGCGAAT	50.0	45.0	58.0	57.9	852 bp	0.333333	52.6