Hotspot

ndhC-trnV-UAC

LSC · 53198-54123 · 926 bp

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Pi 0.00585
MarkerSeek score 63.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
926
variable_sites
8
pi
0.00585
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCTGGCCCTTTGCTAATTGG GCACGTTGGGTCTTTGAAAG 50.0 50.0 58.1 57.9 1065-1139 bp 1.0 82.4
ndhC-trnV-UAC_p2 TCTGGCCCTTTGCTAATTGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.1 57.8 1001-1075 bp 1.0 81.8
ndhC-trnV-UAC_p3 TCTGGCCCTTTGCTAATTGG AATGCACGTTGGGTCTTTGA 50.0 45.0 58.1 58.3 1068-1142 bp 1.0 80.9
ndhC-trnV-UAC_p4 TCTGGCCCTTTGCTAATTGG ATGCACGTTGGGTCTTTGAA 50.0 45.0 58.1 58.3 1067-1141 bp 1.0 80.9
ndhC-trnV-UAC_p5 CCCTTTGCTAATTGGGGCTA GCACGTTGGGTCTTTGAAAG 50.0 50.0 57.5 57.9 1060-1134 bp 1.0 80.0