Hotspot

atpA-atpF

LSC · 12723-12778 · 56 bp

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Pi 0.0606

MarkerSeek score 75.30

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 5
pi: 0.0606
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGTCGCCTACTTGAAGTAC	TGTTTGGGTCGCTGAAAGAA	55.0	45.0	58.1	58.0	200-201 bp	1.0	81.3
atpA-atpF_p2	GTCGCCTACTTGAAGTACGG	TGTTTGGGTCGCTGAAAGAA	55.0	45.0	58.1	58.0	198-199 bp	1.0	81.3
atpA-atpF_p3	CCGTCGCCTACTTGAAGTAC	ATGTTTGGGTCGCTGAAAGA	55.0	45.0	58.1	57.7	201-202 bp	1.0	79.5
atpA-atpF_p4	GTCGCCTACTTGAAGTACGG	ATGTTTGGGTCGCTGAAAGA	55.0	45.0	58.1	57.7	199-200 bp	1.0	79.5
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	CATGTTTGGGTCGCTGAAAG	55.0	50.0	58.1	57.7	200-201 bp	1.0	78.9