Hotspot

ndhK

LSC · 11147-11788 · 642 bp

Back to Campylotropis

Pi 0.00497

MarkerSeek score 44.80

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 642
variable_sites: 22
pi: 0.00497
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	AGGGGGCATTAGAATGGTCT	CAGTTCACTGCTTCATGGGA	50.0	50.0	58.1	57.8	744-759 bp	0.9	76.6
ndhK_p2	GGTTCAGTTTATGCATGGCG	CAGTTCACTGCTTCATGGGA	50.0	50.0	57.8	57.8	766-781 bp	0.9	75.7
ndhK_p3	GTTTATGCATGGCGAAAGGG	CAGTTCACTGCTTCATGGGA	50.0	50.0	57.8	57.8	760-775 bp	0.9	75.5
ndhK_p4	GCGAAAGGGGGCATTAGAAT	CAGTTCACTGCTTCATGGGA	50.0	50.0	58.3	57.8	749-764 bp	0.9	74.8
ndhK_p5	AGGGGGCATTAGAATGGTCT	TCACTGCTTCATGGGAGGAT	50.0	50.0	58.1	58.4	740-755 bp	0.9	74.8