Hotspot

ndhA

SSC · 117801-120173 · 2373 bp

Back to Campylotropis

Pi 0.00608

MarkerSeek score 66.50

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2373
variable_sites: 95
pi: 0.00608
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TGAATCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	37.5	45.0	57.6	56.5	2476-2512 bp	1.0	45.2
ndhA_p2	TGAATCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGAA	37.5	42.9	57.6	57.2	2476-2512 bp	1.0	45.2
ndhA_p3	TCCAGTTACCATAAGGAACA	TGGGAGAAGTTGATCGTTGA	40.0	45.0	53.2	56.5	2472-2508 bp	1.0	45.2
ndhA_p4	ACCATAAGGAACATATCGTGA	TGGGAGAAGTTGATCGTTGA	38.1	45.0	54.2	56.5	2465-2501 bp	1.0	45.2
ndhA_p5	TGAATCCAGTTACCATAAGGAACA	ATGGGAGAAGTTGATCGTTGA	37.5	42.9	57.6	57.0	2477-2513 bp	1.0	45.2