Hotspot

accD

LSC · 56407-57933 · 1527 bp

Back to Campylotropis

Pi 0.004

MarkerSeek score 43.20

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 48
pi: 0.004
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	CCCAATGAGCCGTTTATCGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2316-2639 bp	1.0	86.7
accD_p2	TCCCAATGAGCCGTTTATCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	2317-2640 bp	1.0	86.7
accD_p3	CCCAATGAGCCGTTTATCGA	ACAGCGACCGATAACAATCC	50.0	50.0	58.1	58.1	2689-2981 bp	1.0	86.6
accD_p4	TCCCAATGAGCCGTTTATCG	ACAGCGACCGATAACAATCC	50.0	50.0	58.1	58.1	2690-2982 bp	1.0	86.6
accD_p5	CCCAATGAGCCGTTTATCGA	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	2322-2645 bp	1.0	86.1