Hotspot

rps3-rps19

LSC · 82138-82621 · 484 bp

Back to Campylotropis

Pi 0.0213

MarkerSeek score 84.20

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 56
pi: 0.0213
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAAGTCTGAAACCAAGCGGA	TAAACTTCCGAGGACATGCG	50.0	50.0	58.4	58.0	543-584 bp	1.0	88.6
rps3-rps19_p2	CAAGTCTGAAACCAAGCGGA	TCCGAGGACATGCGAAAAAT	50.0	45.0	58.4	57.9	537-578 bp	1.0	87.9
rps3-rps19_p3	CAAGTCTGAAACCAAGCGGA	TTCCGAGGACATGCGAAAAA	50.0	45.0	58.4	58.1	538-579 bp	1.0	87.8
rps3-rps19_p4	AAGTCTGAAACCAAGCGGAT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	542-583 bp	1.0	87.3
rps3-rps19_p5	AGTCTGAAACCAAGCGGATT	TAAACTTCCGAGGACATGCG	45.0	50.0	57.4	58.0	541-582 bp	1.0	87.3