Hotspot

rps8-rpl14

LSC · 78897-79241 · 345 bp

Back to Campylotropis

Pi 0.0194

MarkerSeek score 75.50

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 345
variable_sites: 33
pi: 0.0194
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	AGCGATGGTATCCTTACCCA	AATCGCCCGGGAATTAAGAC	50.0	50.0	57.9	58.0	433-477 bp	1.0	89.1
rps8-rpl14_p2	AGCGATGGTATCCTTACCCA	CAATCGCCCGGGAATTAAGA	50.0	50.0	57.9	58.0	434-478 bp	1.0	89.1
rps8-rpl14_p3	AGCGATGGTATCCTTACCCA	GCCCGGGAATTAAGACAGTT	50.0	50.0	57.9	57.9	429-473 bp	1.0	88.4
rps8-rpl14_p4	AGCGATGGTATCCTTACCCA	AATCTTTTGTGCAATCGCCC	50.0	45.0	57.9	57.6	445-489 bp	1.0	86.2
rps8-rpl14_p5	CAGCGATGGTATCCTTACCC	AATCGCCCGGGAATTAAGAC	55.0	50.0	57.5	58.0	434-478 bp	1.0	86.2