Hotspot

psaJ-rpl33

LSC · 65297-65790 · 494 bp

Back to Campylotropis

Pi 0.0128

MarkerSeek score 59.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 32
pi: 0.0128
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACATATCTTTCCGTGGCACC	CACAACCGGTACACTCCAAA	50.0	50.0	58.0	58.0	653-687 bp	1.0	86.0
psaJ-rpl33_p2	TTGGCGGGTCTCTTGATAGA	CACAACCGGTACACTCCAAA	50.0	50.0	58.1	58.0	605-639 bp	1.0	85.7
psaJ-rpl33_p3	TACTCTATGGTTCGGGGCTT	CACAACCGGTACACTCCAAA	50.0	50.0	57.8	58.0	624-658 bp	1.0	85.2
psaJ-rpl33_p4	TGGCACCGGTAGTAAGTACT	CACAACCGGTACACTCCAAA	50.0	50.0	57.8	58.0	640-674 bp	1.0	84.6
psaJ-rpl33_p5	GTGGCACCGGTAGTAAGTAC	CACAACCGGTACACTCCAAA	55.0	50.0	57.7	58.0	641-675 bp	1.0	84.1