Hotspot

petA-psbJ

LSC · 61466-62318 · 853 bp

Back to Campylotropis

Pi 0.0113

MarkerSeek score 68.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 51
pi: 0.0113
unique_haplotype_count: 18
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTCCAAGGTCTCTTGTTCT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.2	57.4	989-1009 bp	1.0	74.2
petA-psbJ_p2	GCGTCCAAGGTCTCTTGTTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.9	57.4	990-1010 bp	1.0	74.0
petA-psbJ_p3	TTACGCGTCCAAGGTCTCTT	CATATTCCGGATTGGGCTCA	50.0	50.0	59.0	57.4	994-1014 bp	1.0	72.4
petA-psbJ_p4	CGTCCAAGGTCTCTTGTTCTT	CATATTCCGGATTGGGCTCA	47.6	50.0	57.9	57.4	989-1009 bp	1.0	71.9
petA-psbJ_p5	CGTCCAAGGTCTCTTGTTCT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.2	57.1	987-1007 bp	1.0	71.9