Hotspot

trnG-UCC-trnS-GCU

LSC · 52460-52874 · 415 bp

Back to Campylotropis

Pi 0.0213
MarkerSeek score 68.30
Primer available yes
Label trnG-UCC-trnS-GCU

Feature Metadata

label_name
trnG-UCC-trnS-GCU
feature_type
igs
region
LSC
length_bp
415
variable_sites
46
pi
0.0213
unique_haplotype_count
19
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnS-GCU_p1 ACGAATCGCACTTTTACCACT CAATCCGACGCTTTAGTCCA 42.9 50.0 58.2 58.0 470-510 bp 1.0 81.5
trnG-UCC-trnS-GCU_p2 ACGAATCGCACTTTTACCACT AATCCGACGCTTTAGTCCAC 42.9 50.0 58.2 58.0 469-509 bp 1.0 81.5
trnG-UCC-trnS-GCU_p3 ACGAATCGCACTTTTACCAC AATCCGACGCTTTAGTCCAC 45.0 50.0 56.8 58.0 469-509 bp 1.0 81.5
trnG-UCC-trnS-GCU_p4 ACGAATCGCACTTTTACCAC CAATCCGACGCTTTAGTCCA 45.0 50.0 56.8 58.0 470-510 bp 1.0 81.4
trnG-UCC-trnS-GCU_p5 ACGAATCGCACTTTTACCACT ACGGAAAGAGAGGGATTCGA 42.9 50.0 58.2 58.2 523-563 bp 1.0 79.6