Hotspot

atpA-trnR-UCU

LSC · 51128-51469 · 342 bp

Back to Campylotropis

Pi 0.0155

MarkerSeek score 64.60

Primer available yes

Label atpA-trnR-UCU

Feature Metadata

label_name: atpA-trnR-UCU
feature_type: igs
region: LSC
length_bp: 342
variable_sites: 28
pi: 0.0155
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-trnR-UCU_p1	CATTCACTGGGGAAGCAGAA	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	429-482 bp	1.0	73.9
atpA-trnR-UCU_p2	AAGACATTCACTGGGGAAGC	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.8	57.3	433-486 bp	1.0	73.9
atpA-trnR-UCU_p3	GGAAGCAGAAGCCCTTTTGA	GGTTCAAATCCTATTGGACGC	50.0	47.6	58.4	57.3	419-472 bp	1.0	72.6
atpA-trnR-UCU_p4	ATTCACTGGGGAAGCAGAAG	GGTTCAAATCCTATTGGACGC	50.0	47.6	57.5	57.3	428-481 bp	1.0	71.5
atpA-trnR-UCU_p5	GACATTCACTGGGGAAGCAG	GGTTCAAATCCTATTGGACGC	55.0	47.6	58.5	57.3	431-484 bp	1.0	71.2