Hotspot

atpH-atpF

LSC · 47630-48219 · 590 bp

Back to Campylotropis

Pi 0.013

MarkerSeek score 74.60

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 590
variable_sites: 34
pi: 0.013
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	TGGGCTGGTTGTAGCATTAG	GCCAATAGCCCAAACAAAGG	50.0	50.0	57.6	57.6	633-684 bp	1.0	81.9
atpH-atpF_p2	CCAGAGGCAGAGGGAAAAAT	GCCAATAGCCCAAACAAAGG	50.0	50.0	57.5	57.6	704-755 bp	1.0	80.7
atpH-atpF_p3	TGGGCTGGTTGTAGCATTAG	TTAAACCCGAAACTTCCGGC	50.0	50.0	57.6	58.8	658-709 bp	1.0	78.7
atpH-atpF_p4	TTTATGGGCTGGTTGTAGCA	GCCAATAGCCCAAACAAAGG	45.0	50.0	57.1	57.6	637-688 bp	1.0	78.1
atpH-atpF_p5	CCAGAGGCAGAGGGAAAAAT	TTAAACCCGAAACTTCCGGC	50.0	50.0	57.5	58.8	729-780 bp	1.0	77.5