Hotspot

rps2-atpI

LSC · 45576-45921 · 346 bp

Back to Campylotropis

Pi 0.0335

MarkerSeek score 75.30

Primer available yes

Label rps2-atpI

Feature Metadata

label_name: rps2-atpI
feature_type: igs
region: LSC
length_bp: 346
variable_sites: 27
pi: 0.0335
unique_haplotype_count: 15
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-atpI_p1	TTGTGAAGGTCGTTCTAGCT	CATGCACTTGGAAACTCCCT	45.0	50.0	56.5	58.1	453-504 bp	1.0	76.5
rps2-atpI_p2	TTGTGAAGGTCGTTCTAGCT	GCCAATAGAAATGTTGCCCG	45.0	50.0	56.5	57.8	429-480 bp	1.0	75.7
rps2-atpI_p3	TTGTGAAGGTCGTTCTAGCT	AAGTACTTGGGCATGCACTT	45.0	45.0	56.5	57.7	464-515 bp	1.0	74.7
rps2-atpI_p4	TTTGTGAAGGTCGTTCTAGCT	CATGCACTTGGAAACTCCCT	42.9	50.0	57.3	58.1	454-505 bp	1.0	74.4
rps2-atpI_p5	TTGTGAAGGTCGTTCTAGCT	AGTACTTGGGCATGCACTTG	45.0	50.0	56.5	58.5	463-514 bp	1.0	73.3