Hotspot

psbD-trnT-GGU

LSC · 28715-30112 · 1398 bp

Back to Campylotropis

Pi 0.00992
MarkerSeek score 74.70
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1398
variable_sites
86
pi
0.00992
unique_haplotype_count
20
primer_available
yes
alignment_reliability
0.98
markerseek_score
74.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CACAAAAACGAAACGGTCCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1482-1563 bp 1.0 86.7
psbD-trnT-GGU_p2 CCACAAAAACGAAACGGTCC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1483-1564 bp 1.0 86.7
psbD-trnT-GGU_p3 CCCACAAAAACGAAACGGTC GTGGTAGAGTAACGCCATGG 50.0 55.0 58.2 58.1 1484-1565 bp 1.0 86.7
psbD-trnT-GGU_p4 CACAAAAACGAAACGGTCCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1485-1566 bp 1.0 86.5
psbD-trnT-GGU_p5 CCACAAAAACGAAACGGTCC TCAGTGGTAGAGTAACGCCA 50.0 50.0 58.2 58.1 1486-1567 bp 1.0 86.5