Hotspot

trnG-GCC-psbZ

LSC · 24867-25444 · 578 bp

Back to Campylotropis

Pi 0.0111

MarkerSeek score 68.20

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 578
variable_sites: 37
pi: 0.0111
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	CTCCTGAAGGTTGGTCAAGT	50.0	50.0	57.8	57.1	729-785 bp	1.0	78.4
trnG-GCC-psbZ_p2	CGTCTTCTCCTTGGCAAAGA	CCCGTTGTATTTGCTTCTCC	50.0	50.0	57.8	56.8	745-801 bp	1.0	76.1
trnG-GCC-psbZ_p3	CGTCTTCTCCTTGGCAAAGA	CTTCTCCTGAAGGTTGGTCA	50.0	50.0	57.8	56.8	732-788 bp	1.0	76.0
trnG-GCC-psbZ_p4	CGTCTTCTCCTTGGCAAAGA	TCTCCTGAAGGTTGGTCAAG	50.0	50.0	57.8	56.8	730-786 bp	1.0	76.0
trnG-GCC-psbZ_p5	CGTCTTCTCCTTGGCAAAGA	CCCGTTGTATTTGCTTCTCCT	50.0	47.6	57.8	58.3	745-801 bp	1.0	75.4