Hotspot

trnL-UAA-trnT-UGU

LSC · 14343-15297 · 955 bp

Back to Campylotropis

Pi 0.0116

MarkerSeek score 67.70

Primer available yes

Label trnL-UAA-trnT-UGU

Feature Metadata

label_name: trnL-UAA-trnT-UGU
feature_type: igs
region: LSC
length_bp: 955
variable_sites: 61
pi: 0.0116
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnT-UGU_p1	GCGTCTACCTATTTCGCCAT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	957-1045 bp	1.0	86.6
trnL-UAA-trnT-UGU_p2	GCGTCTACCTATTTCGCCAT	ATCGAGAAAAGCCGGCTATC	50.0	50.0	57.8	57.8	1009-1097 bp	1.0	86.1
trnL-UAA-trnT-UGU_p3	GCGTCTACCTATTTCGCCAT	GGATTCGAACCGATGACCAT	50.0	50.0	57.8	57.8	989-1077 bp	1.0	85.6
trnL-UAA-trnT-UGU_p4	CCCATTGTGAATTCCAGGGT	GCGATGCTCTAACCTCTGAG	50.0	55.0	57.8	57.9	1044-1132 bp	0.95	83.5
trnL-UAA-trnT-UGU_p5	CCCATTGTGAATTCCAGGGT	ATCGAGAAAAGCCGGCTATC	50.0	50.0	57.8	57.8	1096-1184 bp	0.95	83.1