Hotspot

trnF-GAA-trnL-UAA

LSC · 13332-13734 · 403 bp

Back to Campylotropis

Pi 0.0119

MarkerSeek score 61.60

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 30
pi: 0.0119
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TTTGAACTGGTGACACGAGG	AATCGTGAGGGTTCAAGTCC	50.0	50.0	58.1	57.5	485-522 bp	1.0	82.3
trnF-GAA-trnL-UAA_p2	TACCAACTGAGCTATCCCGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	57.6	57.5	447-484 bp	1.0	79.7
trnF-GAA-trnL-UAA_p3	GAACTGGTGACACGAGGATT	AATCGTGAGGGTTCAAGTCC	50.0	50.0	57.5	57.5	482-519 bp	1.0	79.1
trnF-GAA-trnL-UAA_p4	TTTGAACTGGTGACACGAGG	TTCAAGTCCCTCTATCCCCA	50.0	50.0	58.1	57.0	474-511 bp	1.0	78.6
trnF-GAA-trnL-UAA_p5	TCCTCTGCTCTACCAACTGA	AATCGTGAGGGTTCAAGTCC	50.0	50.0	57.4	57.5	457-494 bp	1.0	78.3