Hotspot

ycf23

Genome · 127732-128574 · 843 bp

Back to Campylaephora

Pi 0.0854

MarkerSeek score 78.40

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 843
variable_sites: 72
pi: 0.0854
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	ACGTTGTGAAACTCTTGTCG	GGTTGTAAGCGCTGTGAAAC	45.0	50.0	56.6	58.0	1566-1567 bp	1.0	74.8
ycf23_p2	CGTTGTGAAACTCTTGTCGT	GGTTGTAAGCGCTGTGAAAC	45.0	50.0	56.6	58.0	1565-1566 bp	1.0	74.8
ycf23_p3	ACGTTGTGAAACTCTTGTCGT	GGTTGTAAGCGCTGTGAAAC	42.9	50.0	58.4	58.0	1566-1567 bp	1.0	74.7
ycf23_p4	CGTTGTGAAACTCTTGTCGT	ATGGGATGGCTGTAAAGCTG	45.0	50.0	56.6	57.9	1624 bp	0.5	48.6
ycf23_p5	CGTTGTGAAACTCTTGTCGT	CATGGGATGGCTGTAAAGCT	45.0	50.0	56.6	57.9	1625 bp	0.5	48.6