Hotspot

trpA

Genome · 140382-141173 · 792 bp

Back to Campylaephora

Pi 0.0745

MarkerSeek score 83.30

Primer available yes

Label trpA

Feature Metadata

label_name: trpA
feature_type: gene
region: Genome
length_bp: 792
variable_sites: 59
pi: 0.0745
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpA_p1	CGTCTAGAGGCCTAGGACAT	GGTCGCTAACTCAACGGTAG	55.0	55.0	57.7	58.1	965-973 bp	1.0	81.9
trpA_p2	CGTCTAGAGGCCTAGGACAT	ATGTTGTCAGGGTCGCTAAC	55.0	50.0	57.7	57.9	975-983 bp	1.0	81.8
trpA_p3	TCTCCCTTTCACGGAGGTAA	GGTCGCTAACTCAACGGTAG	50.0	55.0	57.7	58.1	946-954 bp	1.0	81.7
trpA_p4	TCTCCCTTTCACGGAGGTAA	ATGTTGTCAGGGTCGCTAAC	50.0	50.0	57.7	57.9	956-964 bp	1.0	81.6
trpA_p5	CGTCTAGAGGCCTAGGACAT	ACGGTAGAGTACTCGGCTTT	55.0	50.0	57.7	58.2	952-960 bp	1.0	81.4