Hotspot

tilS

Genome · 141326-142309 · 984 bp

Back to Campylaephora

Pi 0.0894

MarkerSeek score 77.40

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: Genome
length_bp: 984
variable_sites: 88
pi: 0.0894
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	CTACCGTTGAGTTAGCGACC	TGCGGGTCCTACAAAAACAA	55.0	45.0	58.1	57.9	1197 bp	1.0	85.1
tilS_p2	GTTAGCGACCCTGACAACAT	TGCGGGTCCTACAAAAACAA	50.0	45.0	57.9	57.9	1187 bp	1.0	85.1
tilS_p3	GTTAGCGACCCTGACAACAT	TGCGCAAAGAACTGGTACTT	50.0	45.0	57.9	58.0	1603 bp	1.0	85.0
tilS_p4	CTACCGTTGAGTTAGCGACC	TGCGCAAAGAACTGGTACTT	55.0	45.0	58.1	58.0	1613 bp	1.0	84.9
tilS_p5	TCCTTTAGCCCCAGCAAATG	TGCGCAAAGAACTGGTACTT	50.0	45.0	58.1	58.0	2061 bp	0.5	58.5