Hotspot

syfB

Genome · 14301-16346 · 2046 bp

Back to Campylaephora

Pi 0.108

MarkerSeek score 77.90

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2046
variable_sites: 220
pi: 0.108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	GAATCCTCCCACCCCAGATA	ACAAAATCCGGCTTATGGCT	55.0	45.0	57.9	57.9	2633-2639 bp	1.0	85.4
syfB_p2	GGGTGTAGCCAAGTGGTAAG	ACAAAATCCGGCTTATGGCT	55.0	45.0	57.9	57.9	2686-2692 bp	1.0	85.3
syfB_p3	GGTGTAGCCAAGTGGTAAGG	ACAAAATCCGGCTTATGGCT	55.0	45.0	57.9	57.9	2685-2691 bp	1.0	85.3
syfB_p4	GAATCCTCCCACCCCAGATA	AGGAAAGTCCGGGCTCTAAT	55.0	50.0	57.9	58.1	2960-2965 bp	1.0	85.1
syfB_p5	GGTGTAGCCAAGTGGTAAGG	TCCGGCTTATGGCTTACTCT	55.0	50.0	57.9	58.2	2679-2685 bp	1.0	84.8