Hotspot

pgmA

Genome · 135490-137058 · 1569 bp

Back to Campylaephora

Pi 0.0841

MarkerSeek score 77.10

Primer available yes

Label pgmA

Feature Metadata

label_name: pgmA
feature_type: gene
region: Genome
length_bp: 1569
variable_sites: 132
pi: 0.0841
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pgmA_p1	GCAATTGCATCAGCTGACAG	TGGCTTCTTTGATGACTGCA	50.0	45.0	58.1	57.7	2916 bp	0.5	57.1
pgmA_p2	TGCATCAGCTGACAGTCAAG	TGGCTTCTTTGATGACTGCA	50.0	45.0	58.2	57.7	2911 bp	0.5	56.2
pgmA_p3	ACACAAATGCCTGGTCCTAC	TGGCTTCTTTGATGACTGCA	50.0	45.0	57.8	57.7	2739 bp	0.5	56.2
pgmA_p4	TTGCATCAGCTGACAGTCAA	TGGCTTCTTTGATGACTGCA	45.0	45.0	57.7	57.7	2912 bp	0.5	55.7
pgmA_p5	CGTTATCAAAGGGCAACAGC	TGGCTTCTTTGATGACTGCA	50.0	45.0	57.7	57.7	2850 bp	0.5	55.7