Hotspot

orf365

Genome · 109518-110615 · 1098 bp

Back to Campylaephora

Pi 0.116

MarkerSeek score 81.80

Primer available yes

Label orf365

Feature Metadata

label_name: orf365
feature_type: gene
region: Genome
length_bp: 1098
variable_sites: 127
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf365_p1	ATATTGCATCTGTGGCCGAG	TGTGGAATGTAGGCTCTGGA	50.0	50.0	58.1	58.0	2208 bp	0.5	58.7
orf365_p2	ATATTGCATCTGTGGCCGAG	TATGCATCTGGCTGGATTCG	50.0	50.0	58.1	57.8	1770 bp	0.5	57.7
orf365_p3	ATATTGCATCTGTGGCCGAG	CCTTTTCAGGATGGCGGATT	50.0	50.0	58.1	58.2	1738 bp	0.5	57.2
orf365_p4	ATCTCTCCATCTGCTGTTGC	TGTGGAATGTAGGCTCTGGA	50.0	50.0	57.7	58.0	2292 bp	0.5	57.0
orf365_p5	ATATTGCATCTGTGGCCGAG	TTCCAAGCTAATGATGCGGG	50.0	50.0	58.1	58.3	1825 bp	0.5	56.5