Hotspot

moeB

Genome · 22528-23610 · 1083 bp

Back to Campylaephora

Pi 0.115

MarkerSeek score 78.80

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1083
variable_sites: 125
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	GAAGGACTTGAACCCTCGAC	ACTTGGTACGACCTGAAGAAG	55.0	47.6	57.9	57.3	1738 bp	0.5	46.0
moeB_p2	GAAGGACTTGAACCCTCGAC	CTTGGTACGACCTGAAGAAGT	55.0	47.6	57.9	57.3	1737 bp	0.5	46.0
moeB_p3	GAGTTGCCTTACCAGCCATT	ACTTGGTACGACCTGAAGAAG	50.0	47.6	58.2	57.3	1975 bp	0.5	45.4
moeB_p4	GAGTTGCCTTACCAGCCATT	CTTGGTACGACCTGAAGAAGT	50.0	47.6	58.2	57.3	1974 bp	0.5	45.4
moeB_p5	TACACGCCTTGAAGGACTTG	CTTGGTACGACCTGAAGAAGT	50.0	47.6	57.8	57.3	1747 bp	0.5	45.4