Hotspot

infB

Genome · 82044-84329 · 2286 bp

Back to Campylaephora

Pi 0.0862

MarkerSeek score 76.10

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2286
variable_sites: 197
pi: 0.0862
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	ACCTTCTGATTGTGCTGACA	AATGCCGAGACGTTCTACAG	45.0	50.0	57.1	57.7	2715 bp	1.0	76.6
infB_p2	GTGCTGACATTCCATCTCTGT	AATGCCGAGACGTTCTACAG	47.6	50.0	58.0	57.7	2704 bp	1.0	76.0
infB_p3	TGTGCTGACATTCCATCTCTG	AATGCCGAGACGTTCTACAG	47.6	50.0	58.0	57.7	2705 bp	1.0	75.9
infB_p4	TCACCTTCTGATTGTGCTGA	AATGCCGAGACGTTCTACAG	45.0	50.0	56.8	57.7	2717 bp	1.0	74.2
infB_p5	TCACCTTCTGATTGTGCTGAC	AATGCCGAGACGTTCTACAG	47.6	50.0	58.2	57.7	2717 bp	1.0	74.2