Hotspot

QQR83_pgt017-ycf4

Genome · 110828-110877 · 50 bp

Back to Campylaephora

Pi 0.17

MarkerSeek score 77.70

Primer available yes

Label QQR83_pgt017-ycf4

Feature Metadata

label_name: QQR83_pgt017-ycf4
feature_type: igs
region: Genome
length_bp: 50
variable_sites: 8
pi: 0.17
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
QQR83_pgt017-ycf4_p1	AATCCGCCATCCTGAAAAGG	TGTGGAATGTAGGCTCTGGA	50.0	50.0	58.2	58.0	487-490 bp	1.0	83.0
QQR83_pgt017-ycf4_p2	CCCGCATCATTAGCTTGGAA	TGTGGAATGTAGGCTCTGGA	50.0	50.0	58.3	58.0	400-403 bp	1.0	82.8
QQR83_pgt017-ycf4_p3	ATCGAACCCGCATCATTAGC	TGTGGAATGTAGGCTCTGGA	50.0	50.0	58.5	58.0	406-409 bp	1.0	81.4
QQR83_pgt017-ycf4_p4	CCATCCTGAAAAGGACACCG	TGTGGAATGTAGGCTCTGGA	55.0	50.0	58.5	58.0	481-484 bp	1.0	80.5
QQR83_pgt017-ycf4_p5	CGAATCCAGCCAGATGCATA	TGTGGAATGTAGGCTCTGGA	50.0	50.0	57.8	58.0	458 bp	0.5	58.2