Hotspot

QQR83_pgt016-QQR83_pgt017

Genome · 110740-110756 · 17 bp

Back to Campylaephora

Pi 0.176
MarkerSeek score 77.80
Primer available yes
Label QQR83_pgt016-QQR83_pgt017

Feature Metadata

label_name
QQR83_pgt016-QQR83_pgt017
feature_type
igs
region
Genome
length_bp
17
variable_sites
3
pi
0.176
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
QQR83_pgt016-QQR83_pgt017_p1 AATCCGCCATCCTGAAAAGG TTCCAAGCTAATGATGCGGG 50.0 50.0 58.2 58.3 107 bp 1.0 77.8
QQR83_pgt016-QQR83_pgt017_p2 AATCCGCCATCCTGAAAAGG GCTAATGATGCGGGTTCGAT 50.0 50.0 58.2 58.5 101 bp 1.0 76.6
QQR83_pgt016-QQR83_pgt017_p3 AATCCGCCATCCTGAAAAGG CCAAGCTAATGATGCGGGTT 50.0 50.0 58.2 58.6 105 bp 1.0 75.6
QQR83_pgt016-QQR83_pgt017_p4 CCATCCTGAAAAGGACACCG TTCCAAGCTAATGATGCGGG 55.0 50.0 58.5 58.3 101 bp 1.0 75.4
QQR83_pgt016-QQR83_pgt017_p5 AATCCGCCATCCTGAAAAGG AATGATGCGGGTTCGATTCC 50.0 50.0 58.2 58.7 98 bp 1.0 75.1