Hotspot

rps15

SSC · 115550-115822 · 273 bp

Back to Campsis

Pi 0.446

MarkerSeek score 77.40

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: SSC
length_bp: 273
variable_sites: 115
pi: 0.446
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	AGCAGCCAAGTTTCACATGA	TCCCAACGCGTTACATAAGG	45.0	50.0	58.0	58.0	1177 bp	0.5	59.8
rps15_p2	AGCAGCCAAGTTTCACATGA	CCGAGTCATTCTTCCATGCA	45.0	50.0	58.0	58.0	956 bp	0.5	59.7
rps15_p3	GCAGCCAAGTTTCACATGAC	TCCCAACGCGTTACATAAGG	50.0	50.0	57.9	58.0	1176 bp	0.5	59.4
rps15_p4	AGCAGCCAAGTTTCACATGA	AACACCATGCATTGATGGGT	45.0	45.0	58.0	58.1	1040 bp	0.5	59.4
rps15_p5	CAGCAGCCAAGTTTCACATG	TCCCAACGCGTTACATAAGG	50.0	50.0	57.9	58.0	1178 bp	0.5	59.4