Hotspot

ndhI

SSC · 119364-119870 · 507 bp

Back to Campsis

Pi 0.511

MarkerSeek score 81.70

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 507
variable_sites: 247
pi: 0.511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	CTCTAGCCGAGTGTGAGAGA	TGAAAGGGTGGTGTGGATTG	55.0	50.0	58.0	58.0	1255 bp	0.5	59.7
ndhI_p2	CTCTAGCCGAGTGTGAGAGA	TGAATGAAAGGGTGGTGTGG	55.0	50.0	58.0	58.0	1259 bp	0.5	59.7
ndhI_p3	TCTCTAGCCGAGTGTGAGAG	TGAAAGGGTGGTGTGGATTG	55.0	50.0	58.0	58.0	1256 bp	0.5	59.7
ndhI_p4	TCTCTAGCCGAGTGTGAGAG	TGAATGAAAGGGTGGTGTGG	55.0	50.0	58.0	58.0	1260 bp	0.5	59.7
ndhI_p5	TGTTCCGGAGCTTTTTGACA	TGAAAGGGTGGTGTGGATTG	45.0	50.0	58.0	58.0	1059 bp	0.5	59.6