Hotspot

ndhH

SSC · 115919-117100 · 1182 bp

Back to Campsis

Pi 0.449

MarkerSeek score 79.80

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 432
pi: 0.449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GAAAACGTCAACGACTGCTG	TGAAGAATTCCCAAAGGCCC	50.0	50.0	58.0	58.1	1601 bp	0.5	59.5
ndhH_p2	GAAAACGTCAACGACTGCTG	GCTATGGAGGGACCGATACT	50.0	55.0	58.0	58.1	1694 bp	0.5	59.4
ndhH_p3	GAAAACGTCAACGACTGCTG	CTGCTATGGAGGGACCGATA	50.0	55.0	58.0	58.1	1696 bp	0.5	59.4
ndhH_p4	TTGGGAAAACGTCAACGACT	TGAAGAATTCCCAAAGGCCC	45.0	50.0	58.0	58.1	1605 bp	0.5	59.3
ndhH_p5	TGCATGGAAGAATGACTCGG	TGAAGAATTCCCAAAGGCCC	50.0	50.0	58.0	58.1	1433 bp	0.5	59.3