Hotspot

ndhG

SSC · 120220-120750 · 531 bp

Back to Campsis

Pi 0.517

MarkerSeek score 74.20

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 183
pi: 0.517
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CAATCCACACCACCCTTTCA	AATAGCCGGGCCAATAGTTG	50.0	50.0	58.0	58.0	1115 bp	0.5	59.8
ndhG_p2	CAATCCACACCACCCTTTCA	CAATAGCCGGGCCAATAGTT	50.0	50.0	58.0	58.0	1116 bp	0.5	59.8
ndhG_p3	CCACACCACCCTTTCATTCA	AACAATAGCCGGGCCAATAG	50.0	50.0	58.0	58.0	1114 bp	0.5	59.8
ndhG_p4	CCACACCACCCTTTCATTCA	AATAGCCGGGCCAATAGTTG	50.0	50.0	58.0	58.0	1111 bp	0.5	59.8
ndhG_p5	CCACACCACCCTTTCATTCA	CAATAGCCGGGCCAATAGTT	50.0	50.0	58.0	58.0	1112 bp	0.5	59.8