Hotspot

ndhF

SSC · 126367-128604 · 2238 bp

Back to Campsis

Pi 0.46

MarkerSeek score 77.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2238
variable_sites: 819
pi: 0.46
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AAAGAGGTAAGGTCCGGTGA	ATGCCGTCACCCATTCTTAC	50.0	50.0	58.0	58.0	2672 bp	0.5	59.5
ndhF_p2	AAGAGGTAAGGTCCGGTGAA	ATGCCGTCACCCATTCTTAC	50.0	50.0	58.0	58.0	2671 bp	0.5	59.5
ndhF_p3	AAAGAGGTAAGGTCCGGTGA	AAGTGAGGAAGAAAGCGTGG	50.0	50.0	58.0	58.1	2610 bp	0.5	58.9
ndhF_p4	AAGAGGTAAGGTCCGGTGAA	AAGTGAGGAAGAAAGCGTGG	50.0	50.0	58.0	58.1	2609 bp	0.5	58.9
ndhF_p5	AAGAGGTAAGGTCCGGTGAA	GTGAGGAAGAAAGCGTGGAA	50.0	50.0	58.0	58.1	2607 bp	0.5	58.9