Hotspot

ndhD

SSC · 121880-123400 · 1521 bp

Back to Campsis

Pi 0.475

MarkerSeek score 81.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1521
variable_sites: 639
pi: 0.475
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GATACCCTGGGACGGATGTA	TTTGTGTGAACTGGCGAGAA	55.0	45.0	58.0	58.0	1979 bp	0.5	59.7
ndhD_p2	AACTATTGGCCCGGCTATTG	TTTGTGTGAACTGGCGAGAA	50.0	45.0	58.0	58.0	2397 bp	0.5	59.6
ndhD_p3	CAACTATTGGCCCGGCTATT	TTTGTGTGAACTGGCGAGAA	50.0	45.0	58.0	58.0	2398 bp	0.5	59.6
ndhD_p4	CTATTGGCCCGGCTATTGTT	TTTGTGTGAACTGGCGAGAA	50.0	45.0	58.0	58.0	2395 bp	0.5	59.6
ndhD_p5	GATACCCTGGGACGGATGTA	TTGGAGTTGGGACCCAAAAG	55.0	50.0	58.0	57.9	2257 bp	0.5	59.3