Hotspot

ccsA

SSC · 123649-124626 · 978 bp

Back to Campsis

Pi 0.471

MarkerSeek score 81.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 397
pi: 0.471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCAGTAGCTTTTCGATGGCC	GCGGCATGTCATCTCCTAAA	50.0	50.0	58.0	58.0	1686 bp	0.5	59.4
ccsA_p2	CAGTAGCTTTTCGATGGCCT	GCGGCATGTCATCTCCTAAA	50.0	50.0	58.0	58.0	1685 bp	0.5	59.4
ccsA_p3	TTCTCGCCAGTTCACACAAA	GCGGCATGTCATCTCCTAAA	45.0	50.0	58.0	58.0	1151 bp	0.5	59.4
ccsA_p4	TCAGTAGCTTTTCGATGGCC	GAGTTCAAGACAAGCCGCTA	50.0	50.0	58.0	57.9	1773 bp	0.5	59.2
ccsA_p5	CAGTAGCTTTTCGATGGCCT	GAGTTCAAGACAAGCCGCTA	50.0	50.0	58.0	57.9	1772 bp	0.5	59.1